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KMID : 0617320000090010069
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Journal of Pharmacetical Sceiences Ewha Womans University
2000 Volume.9 No. 1 p.69 ~ p.70
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Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A
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Min, Kyeongsik
Kim, Sun Young/Song, Hyun Kyu/Chang, Changsoo/Cho, Seung-Je/Moon, Jinho/Yang, Jin Kuk
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Abstract
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Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P2_1, with unit-cell parameters a = 74.21. b = 78.11, c = 82.29 Å, β = 101.33°. The asymmetric unit contains a homohexamer. with a corresponding crystal volume per protein mass (V_m) of 2.27 Å^3 Da^-1 and a solvent content of 46%. Native X-ray data to 2.15 Å resolution have been collected using synchrotron X-rays.
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